Milestones of the first year of iMeta. iMeta is an open-access Wiley partner journal launched by iMeta Science Society consisting of worldwide scientists in bioinformatics and metagenomics. In 2022, iMeta released four issues, including 60 publications with a total of 340 citations. iMeta has been indexed in several databases, including Google Scholar, Crossref, CNKI, Dimensions, PubMed (partial), DOAJ, and Scopus. Thanks to the editorial board members and reviewers for their contributions to the iMeta in 2022.

We used surface soils collected from 237 sites across the globe and analyzed how environmental conditions affect soil bacterial occurrences and genome size. We used a joint species distribution model to quantify the effects of environments on species occurrences and found that aridity was a major regulator of genome size with warmer and drier environments selecting bacteria with smaller genomes.

We concluded that the macroecological theory could be applied to soil microbial life-history traits and also highlight the key effects of plant and soil properties on the changes in microbial strategies. These findings were suitable for this Journal (significantly advance the field of Microbial Ecology) and first proved that macroecological theory could be applied to soil microbial community, which was benefit to the development of microbial ecology and can be regarded as the evidence for most of researches.

We developed a cuproptosis activity score to reflect the overall cuproptosis level, which could provide a methodological reference for future cuproptosis studies. Cuproptosis is associated with a variety of tumor-related pathways, and in particular, tumors with high fatty acid metabolism may be more sensitive to cuproptosis. Cuproptosis is associated with the regulation of the tumor microenvironment and can predict the outcome of immunotherapy patients based on cuproptosis activity. This study identified cuproptosis-related microRNAs, long noncoding RNAs, and transcription factors in each tumor type, which can provide a resource and reference for future cuproptosis-related research.

Environmental DNA (eDNA) is a quick and accurate method for biodiversity assessment. This study first used eDNA-assessed regional plant species diversity. Temperature instead of precipitation is the main factors affecting plant diversity. Human activities were proven to affect plant species diversity in desert areas. More instant plant diversity results need to be done for desertification control.

Quantifying the contributions of possible environmental sources (“sources”) to a specific microbial community (“sink”) is a classical problem in microbiology known as microbial source tracking (MST). We show compelling evidence that solving the MST problem using existing MST solvers is impractical when ecological dynamics plays a role in community assembly. In particular, we clearly demonstrate that the presence of either microbial interactions or priority effects will render the MST problem mathematically unsolvable for MST solvers.

This study revealed that, with increasing salinity, the microbial diversity declined, the taxonomic structure underwent marked turnover, and the complexity and stability of ecological networks diminished. In low-stress conditions, the abundant biosphere played a more important role than the rare biosphere in stabilizing ecological networks, but the difference between their relative importance narrowed significantly with the increasing stress.

In this study, a 33-year fertilization experiment was conducted to investigate the changes in soil BNF rates, diazotrophic and AMF communities, and their interactions after various long-term fertilizations. We found a remarkable increase in soil BNF rates after more than three decades of fertilization compared with nonfertilized soil, and the green manure treatment rendered the highest enhancement.

Multiple bacterial pathogen detection (MBPD) provides the accurate and comprehensive detection of bacterial pathogens from biological and environmental samples based on 16S rRNA gene sequencing. By constructing a relatively complete database of 72,685 sequences for wide identification across a broad range of bacterial pathogens causing animal, plant, and zoonotic diseases, MBPD can provide the appropriate threshold for common variable regions of 16S rRNA gene in taxonomy alignment based on the recommendation of in silico experiment. MBPD is freely available at https://github.com/LorMeBioAI/MBPDLorMeBioAI/MBPD.

EasyAmplicon is a user-friendly, cross-platform, and community-supported pipeline for amplicon data analysis. It has most of the modules for data processing and visualization in microbiome research. The pipeline is maintained and updated regularly. We encourage users to contribute appropriate code.

Gut microbiota dysbiosis increases the host's susceptibility to diseases. Identified characteristic microbes in high-fat diet (HFD) induced obesity, diabetes, gastrointestinal diseases, neurodegeneration, and cardiovascular diseases. Bile acids, lipopolysaccharide, short-chain fatty acids, and trimethylamine N-oxide are the commonalities in HFD-induced chronic diseases. FXR, TGR5, NF-κB, PPAR-γ, and PERK signaling were critical for the interacted mechanisms toward microbiota modulation. Provided new insights into mechanisms among microbiota, metabolites, and immune responses in chronic diseases.

Strategies and tools in illumina and nanopore-integrated metagenomic analysis of microbiome data.

In this review, we have systematically summarized the advances of multi-omics and their existing and potential applications in tumor microbiome research, thus providing an omics toolbox for investigators to reference in the future.

Immune T cells are involved in metabolic-associated fatty liver disease (MAFLD) progression either in a positive or negative manner and the therapeutic methods of MAFLD caused by disordered T cells include gut microbiota regulation, adoptive cell transfer, gene editor microRNAs, drugs (inhibitors/traditional Chinese medicine) therapy.

A new release of PhyloSuite, capable of conducting tree-based analyses. Detailed guidelines for each step of phylogenetic and tree-based analyses, following the “What? Why? and How?” structure. This protocol will help beginners learn how to conduct multilocus phylogenetic analyses and help experienced scientists improve their efficiency.

The article provides a pipeline for comparing microbial co-occurrence networks based on the R microeco package and meconetcomp package. It has high flexibility and expansibility and can help users efficiently compare networks built from different groups of samples or different construction approaches.

The human-originated Lachnospiraceae biobank included 77 species was constructed. In vitro metabolite profiling of 110 Lachnospiraceae strains yielded 242 metabolites of 17 categories. Many Lachnospiraceae strains produce short-chain fatty acids, and Agathobacter rectalis strain Lach-101 and Coprococcus comes strain NSJ-173 are the top two butyric acid producers in vitro.

A new method, quantification of microbial absolute abundance differences (QMD), was proposed to estimate the microbial absolute abundance changes of each taxon under different conditions based on the microbial relative abundance. Compared with other methods, QMD displayed greater confidence in understanding microbiome dynamics between groups. We also provide QMD software to investigate common deviations and achieve a better understanding of microbiota changes under different conditions.

OmicStudio focuses on speed, quality together with flexibility. Generally, OmicStudio can not only meet the users' demand of ordinary bioinformatics data analysis, statistics, and visualization, but also provides them freedom of data mining beyond developer's framework. Additionally, unlimited to developer's aesthetics, users can get more elegant graphs through customizing. Available online https://www.omicstudio.cn.

Aggravated algal blooms potentially decreased environmental heterogeneity. Different strategies of planktonic bacteria and eukaryotes in response to aggravated algal blooms. Environmental constraints of plankton showed different patterns over time.

It has long been recognized that pH mediates community structure changes in glacier foreland soils. Here, we showed that pH changes resulted from plant colonization. Plant colonization reduced pH and increased soil organic carbon, which increased bacterial diversity, changed the community structure of both bacteria and fungi, enhanced environmental filtering, and improved microbial network disturbance resistance.